Estudio de lA dinámica del cilio primario y del canal de
potasio Kv10.1 mediante microscopía confocal

José Manuel Sánchez, Irene Carretero, Jorge González, Diana Urrego, Walter Stühmer and Luis A. Pardo
Max Planck Institute for Experimental Medicine, Göttingen, Germany.

OBJEC TIVES : It is believed that tumor cells expressing Kv10.1 channel acquire selective advantages that allow them to maintain chronic proliferation. The ciliary disassembly is a prerequisite to enter into the cell cycle, therefore our objective was to find correlations between primary cilium disassembly and the Kv10.1 channel through the Kv10.1 cortactin binding site.

Methods: Wild type Kv10.1, as well as the mutant form lacking the binding site to the cytoskeleton protein cortactin, were overexpressed in RPE -TERT cell line. The effect on ciliary disassembly of such overexpression forms was imaging using confocal microscopy.

Results: Kv10.1 wild type overexpression induced disassembly of the primary cilium and alteration of the cytoskeleton, which corresponded to overproduction of filament structures positive to α-tubulin acetylated in the cytoplasm. Overexpression of mutated Kv10.1 did not show this pattern, nor the untreated cells.

ConclusionS: This data suggests that Kv10.1 channel could be interfering with the cilium formation, and thus influences the cell cycle in a way that cortactine binding site is not involved. That should be furtherly studied because due to its possible implications in cancer.

Keywords: Kv10.1 channel, primary cilium, cortactin, acetylated alpha-tubulin.
Palabras clave: canal Kv10.1, cilio primario, cortactina, alfa-tubulina acetilada.

Descargar “4 – amu4 – Primary cilium, cortactine, and Kv10.1 dynamics under confocal microscopy” 4-amu4-Primary-cilium-cortactine-and-Kv10.1-dynamics-under-confocal-microscopy.pdf – Descargado 116 veces – 546 KB